Penetration of sperm from teratospermic men into zona-free hamster eggs
نویسندگان
چکیده
منابع مشابه
Preliminary study of buffalo sperm penetration into zona-free hamster eggs after treatment with calcium ionophore A23187.
A previous study (Takahashi & Hanada, 1984) reported the penetration of zona-free hamster eggs by ejaculated bull spermatozoa after treatment with calcium ionophore A23187 (Ca-IA) in the presence of caffeine. Based on this study, successful IVF was accomplished not only in cattle (Hanada, 1986b) but also in goats and sheep (Hanada, 1986a) using spermatozoa treated with Ca-IA. In the present stu...
متن کاملZona-free hamster ovum penetration by human spermatozoa: influence of various sperm factors.
The present paper evaluates the influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova. Higher in vitro fertilization rates were obtained after sperm selection by "swim-up" migration, 4-h incubation, sperm dilution to a concentration of 2.5 X 10(6) spermatozoa/ml, and observation of the oocytes 8 h after insemina...
متن کاملEffects of white blood cells on the in vitro penetration of zona-free hamster eggs by human spermatozoa.
The presence of white blood cells in semen has been associated with male infertility. Previous studies indicate that pyospermia occurs in conjunction with decreases in sperm motility, number of normal sperm forms, and penetration rates in the zona-free hamster egg sperm penetration assay. We have evaluated the relationship of seminal white blood cells and sperm function, as reflected in the zon...
متن کاملRelationship between human in-vitro fertilization and intracytoplasmic sperm injection and the zona-free hamster egg penetration test.
The zona-free hamster egg penetration test (HEPT) is widely used for evaluating the fertilizing ability of human spermatozoa. However, the relationship between the HEPT and microassisted fertilization has yet to be determined. To evaluate the efficiency of HEPT in selecting the most appropriate method of in-vitro fertilization (IVF), including intracytoplasmic sperm injection (ICSI) in couples ...
متن کاملIL-7 and IL-8 inhibit gamete interaction in the zona-free hamster egg sperm penetration assay
Current thought in reproductive endocrinology suggests hat endometriosis-associated subfertility may be the result of an adverse influence of activated immunocompetent cells on fertilization and embryo development. Inflammatory ediators such as interleukin-1 and tumour necrosis actor have been implicated in the pathophysiology of this process. The purpose of this study was to assess the effect ...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1985
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1985.tb00842.x